OZ BIOSCIENCES développe et commercialise une large gamme de produits focalisée sur le transport des molécules biologiquement actives (ADN, siARN, mRNA, protéines anticorps …). Expert dans la transfection et transduction, OZ Biosciences propose plusieurs technologies à bases de lipides, polymères et nanoparticules magnétiques permettant d’offrir une solution adaptée à chaque modèle biologique.

La Magnetofection idéal pour la transfection de cellules primaires ou difficiles à transfecter et pour augmenter l’efficacité de transduction (>1000 publications)
La lipofection et polyfection pour la transfection d’acides nucléiques ou de protéines dans des lignées cellulaires
La transfection en 3D adapté a tous types de matrices 3D
Des outils pour les applications virales, les cellules souches, l’immunisation/adjuvants, des kits d’’analyses…

Venez découvrir notre site internet www.ozbiosciences.com, présentant l’ensemble de nos produits et nos engins de recherche (reagent finder, citations finder, cell database…)

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OZ Biosciences is a product development company that creates, develops and commercializes delivery systems of bioactive materials (DNA, siRNA, proteins, antibodies...), intended for the worldwide scientific community and pharmaceutical industries. Our mission is to assist scientists to accelerate the rhythm of discoveries by offering them technologically advanced tools such as transfection reagents and other life science research products. Principal innovations are based on proprietary technologies such as Magnetofection (magnetic-assisted transfection or infection) and Lipofection (lipid-based transfection), but also mRNA and lipids-based delivery systems (LNP).

With over 20 years of nucleic acid delivery expertise, OZ Biosciences is confident in providing you with the best-in-class services for high quality mRNAs and LNPs production.

We can synthesize custom mRNAs (from gene synthesis to IVT production) at microgram to multigram scales, from a few hundred up to several thousand bases, with diverse modifications including 5’ terminal modifications with cap, internal modifications such as 5-methoxyuridine or N1-methyl-pseudouridine (other modified nucleotides available) and 3’ modifications such as poly-A tail. Fluorescent labeling with Cy5 and others are also available.

We offered also a Microfluidics Platform for the reproducible manufacturing of safe & potent lipid nanoparticles (LNPs). We can support every stage of LNP formulation needs, from design to production. Suitable for any RNA, DNA or APIs, you can provide us with your molecule of interest and we will formulate it into LNPs composed by our unique patented ionizable lipids (licensing available).

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NeuroMag

NeuroMag Transfection Reagent is the first dedicated Magnetofection™ transfection reagent for neurons from 1 DIV to 21 DIV. It has proven to be extremely efficient in transfecting a large variety of primary neurons such as cortical, hippocampal, dorsal root ganglion and motor neurons with all types of nucleic acids. Moreover, high transfection efficiency was also achieved in primary astrocytes, oligodendrocyte precursors or neural stem cells as well as other cell lines (C6, B65, PC12, N2A...). Due to its unique properties, NeuroMag allows following transfected neurons during several days.

"High transfection efficiency on primary dopaminergic neurons at 21 DIV." Underhill SM et al, Neuron. 2014
“Achieve up to 30% of transfection efficiency on primary cortical neurons." Wang R et al, Neurobiol Dis. 2014

NeuroMag Transfection Reagent main benefits:
•    Great efficiency, ideal for primary neurons
•    Efficient from 0 DIV to 21 DIV
•    Nontoxic and completely biodegradable: high transfected neurons viability
•    Ready-to-use, straightforward and rapid
•    For all types of nucleic acids

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GlialMag

Glial-Mag is designed to reach optimal transfection efficiency with microglial cell lines and primary cells. Glial-Mag kit is the association of a specific magnetic nanoparticles formulation (Glial-Mag reagent), issued from our Magnetofection™ technology and a booster (Glial-Boost) designed to enhance transfection efficiency.
Glial-Mag transfection reagent main benefits:
•    Highly efficient with microglial cell lines & primary cells
•    Low nucleic acid amount - minimized toxicity
•    High level of nucleic acid compaction
•    Easy and straightforward protocol
•    Compatible with any culture medium.

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ViroMag

ViroMag Transduction Reagent is a magnetic nanoparticles formulation dedicated to increase virus infection and transduction capacities; it is suitable for all viruses. Based on the Magnetofection™ technology, this reagent allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement of virus transduction with extremely low vector doses. Due to its specific properties, ViroMag is ideal to infect non permissive cells. This reagent demonstrates an exceptionally high efficiency to promote, control and assist viral transductions so that no molecular biology processes or biochemical modifications are required.

ViroMag Transduction reagent main benefits:
•    Increases transduction efficiency
•    Accelerates the transduction process
•    Efficient for hard-to-infect and non-permissive cells
•    Concentrates the viral dose onto the cells
•    Suitable for all type of virus
•    Synchronise cells adsorption/infection without modifications of the viruses

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LentiBlast Premium Transduction Enhancer

LentiBlast Premium is a novel patented chemical composition that dramatically increases lentiviral infection and transduction efficiency in any type of cells, adherent or in suspension, primary or cell lines.

The properties of this reagent allow simultaneously neutralizing electrostatic repulsions between membrane and viral particles and enhancing viral fusion with cell membrane. Due to a favorable “membrane permeable effect” limiting the transmembrane potential changes, LentiBlast Premium is non-toxic and totally compatible with cell viability.

LentiBlast Premium Transduction Enhancer overcomes obstacles that prevent successful transduction (cell density, passage number, lentivirus purity, MOI, ...) and allows you to complete efficient transduction efficiencies without Polybrene.
LentiBlast Premium Transduction Enhancer main benefits:
•    Increase lentivirus infection efficiency for hard-to-transduce cells: The right solution for Stem cells, Primary T cells
•    Save effort & money: Achieve same transduction results with a lower viral titer
•    Maximize Cell Viability: Non-toxic

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HYVIR™ Transfection Reagent

HYVIR™, is a novel lipid-based transfection kit dedicated to enhance viral particles production in HEK-293T.
Its specific formulation allows an optimal plasmid compaction, protection and delivery rendering HYVIR ideal for the production of last generation of lentivirus.
3rd generation system while improving the safety of the 2nd generation by splitting the packaging system into two plasmids also increases the difficulty for delivery: now 4 plasmids need to be transfected at the same time under a precise stoichiometry.
Thanks to its chemical moieties, HYVIR™ is the perfect transfection reagent dedicated to this task as it allows maximal compaction for an efficient co-transfection of packaging plasmid(s), envelope plasmid and lentiviral transfer plasmid encoding your gene of interest.

HYVIR™ Transfection Reagent main benefits:
•    Performant: reach high-titer functional lentiviral / virus production.
•    Highly efficient: co-transfection of 4 plasmids at the same time.
•    Reliable & Reproducible: easily scalable for production in large volumes and can be raised to superior grade.
•    Ideal reagent for HEK293T cells transfection.
•    Completely animal origin free.

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Helix-IN™ Transfection Reagent

Helix-IN™ DNA Transfection Reagent opens up new possibilities for addressing issues of classical transfection technologies.
OZ Biosciences revolutionizes Polyfection with the design of Helix-IN™, a novel patented Cationic Hydroxylated Amphiphilic Multi-block Polymer (CHAMP™ Technology). This novel bi-functional co-polymer is biocompatible, ionizable, pH responsive and biodegradable.
Cellular stress is reduced, viability preserved and efficiency enhanced.

Helix-IN Transfection Reagent main benefits:
•    Broad Spectrum transfection Reagent for cell lines and hard-to-transfect cells
•    High Intracellular Protein Production while preserving viability
•    High secreted Protein Production while minimizing cellular stress
•    Biodegradable - Avoid secondary effects
•    Compatible with any culture medium

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SilenceMag

SilenceMag siRNA Delivery Reagent is based on the Magnetofection™ technology. Simple, rapid and easy to use, SilenceMag is serum compatible and nontoxic. Specifically designed for siRNA transfection, SilenceMag gives reliable high protein knockdown at very low doses of siRNA in numerous cell types (primary cells, hard-to-transfect & cell lines).

SilenceMag siRNA Delivery Reagent main benefits:
•    High protein knockdown efficiency
•    For all siRNA applications
•    No off-target effects
•    Serum compatible & Non toxic
•    Simple, rapid and easy-to-use

"68% of HUVEC were efficiently transfected" Dou L et al, J Am Soc Nephrol. 2014 Aug 21

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HYPE-293™ Transfection Kit

HYPE-293™ Transfection Kit is dedicated to achieve High Yield Protein Expression in HEK-293, 293-S, 293-F or any 293-related cells growing in suspension.
Scale-up to larger volumes for production of milligrams of protein per liter of cell culture is straightforward and easy with simple and cost efficient handling steps.

HYPE-293™ Transfection Kit main benefits:
•    Reach optimal Protein & Antibody production yield
•    Suitable for all HEK293 cells growing in suspension
•    Compatible with any synthetic or regular media used for protein production
•    Ideal for bioreactor, spinner or flasks
•    Animal origin free

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mRNA Ready To Use and Custom Service

OZ Biosciences offers mRNAs that mimic fully processed mature mRNAs. These mRNAs are stabilized with 5’ Cap 1 structure and 3’ poly(A) tail and are optimized to yield improved stability & performance. They are modified with 5-methoxyuridine (5moU replaces U) or N1-methyl-pseudouridine (N1-mψ) to reduce innate immune responses. We also offer unmodified mRNAs.

5 categories of ready-to-use mRNAs :
•    Reporter gene mRNAs: ideal controls to study transfection efficiency.
•    Genome editing mRNAs: Cas9 mRNA for CRISPR Genome editing.
•    Vaccine mRNAs: ideal as controls for immunization or vaccine studies.
•    Gene Replacement mRNAs: EPO mRNA
•    Fluorescent mRNAs: Cy5 GFP mRNA, Cy5 GFP mRNA
•    Interleukin mRNA : H IL-2 mRNA

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LipidNanoParticles (LNP) Ready To Use and Custom Service

The RNA therapeutics have the potential to revolutionize medicinal fields as they are safe, easy to reproduce (e.g. IVT) and offer a great deal of versatility.
Naked mRNA therapeutics are generally unstable. They require high doses and show low permeability to cell membranes. Drug delivery systems are necessary to deliver mRNA for in vitro, pre-clinical and clinical applications. For this purpose, OZ Biosciences provides portfolio & custom lipid nanoparticles (LNP) delivery systems.
To date, formulation in LNP represents the most advanced non-viral delivery platform for nucleic acid therapy and promising candidates to treat manifold diseases.
Watch our Youtube Video to learn more about our LNP synthesis workflow.

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